To determine if MCF-7 cells cultured in 3D maintain their estrogen responsiveness, microtissues were treated with 17β-estradiol for 8 hours, and gene
After, MCF-7 and MCF-7 MS cells were washed with cold PBS (4°C) and resuspended in RIPA
(a) The
These
Injectable estradiol valerate (10 mg/ml) was used as a
Add 22 ml of media to each new flask (we split 1 flask into 3 flasks) 8
Starve the cells Grow the cells to 80% confluence
98-fold over controls (UCL)
BACKGROUND Xenografts of various human cancers in nude mice provide a helpful model in cancer research
Epidemiologic studies and data from National Quality Registers demonstrate a higher incidence of cataract extraction in women [1,2]
Effect of conditioned serum-free media from MCF-7 cells on the induction of DNA-synthesis by IGF-I and E2 in the three MCF-7 strains
To determine whether AG825 can also inhibit the growth of established tumors, another xenograft protocol was used
It is 45 years since a pleural effusion from a patient with metastatic breast cancer led to the generation of the MCF-7 breast cancer cell line
MCF-7 cells are a slow growing estrogen receptor (ER) positive human breast cancer cell line that is commonly used to model estrogen responsive breast cancer cell growth in-vitro and tumour growth in-vivo
After12-18 h, the Red phenol medium is OHT-induced growth inhibition and cell death in MCF-7 cells
This study was undertaken to investigate the
Once MCF-7 cell layer is dispersed (3min at 37°C) deactivate Trypsin by adding 5 ml cells/Trypsin-EDTA to 10mL of complete growth medium (see step 1) in sterile tube
Moreover, the treatment outcomes of breast cancer are affected by the expression of hormone receptors, such as ESR1 and human epidermal growth factor receptor 2 MCF-7, an estrogen-sensitive human breast cancer cell line, was obtained from the RIKEN BioResource Center (Ibaraki, Japan)